Canadian Society for Medical Laboratory Science (CSMLS) Practice Exam

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Which backup test is used to confirm a positive protein in urine?

Ictotest
SSA test
Clinitest
Acetest

The correct answer is: SSA test

The SSA test, or Sulfosalicylic Acid test, is a confirmatory test for the presence of protein in urine. When a urine sample tests positive for protein through initial screening methods, the SSA test serves as a reliable backup test that can confirm the presence and concentration of proteins in the sample. This test is based on the precipitation of proteins when sulfosalicylic acid is added to the urine. The resulting turbidity correlates with the amount of protein present, allowing for qualitative and semi-quantitative assessment. It is particularly useful in detecting non-albumin proteins, which may not be adequately assessed by other methods. While the Ictotest, Clinitest, and Acetest are used for different purposes—such as testing for bilirubin, glucose, and ketones respectively—they do not specifically target protein confirmation in urine. Therefore, their roles do not align with the need to verify protein presence, making the SSA test the appropriate choice for this context.